Fig 1: ATR partially decreased the incidences of T2D in db/db mice. A, Serum insulin level of db/db mice was notably higher than other mice. B, Results of blood glucose tested after injection of 2 g/kg bodyweight during 2 h in glucose tolerance test. The increasing of blood glucose level in db/db mice was relieved by ATR administration. C, Malate concentration detected by LC-MS. Malate concentration was down-regulated in T2D mice and got retrieved to baseline level with ATR treatment. D, The protein expression level of ME1, ME2 and MDH1 in C57BL/6J mice, db/db mice and db/db mice treated with ATR. Relative protein expression levels of ME1, ME2 and MDH1 were significantly down-regulated in db/db mice compared with the control groups, which was partially rescued to normal expression level with ATR treatment. Data = mean ±SD, n = 5 in each group, *P < .05, **P < .01, ***P < .001, compared with NC (C57BL/6J) group. # P < .05, ## P < .01, compared with db/db group